Determination of Paeonolum in Fufang Yuanhu Zhitong Pian by HPLC
- VernacularTitle:高效液相色谱法测定复方元胡止痛片中丹皮酚的含量
- Author:
Yongzhi DING
- Publication Type:Journal Article
- Keywords:
Fufang Yuanhu Zhitong Pian;
Paeonolum;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for detemining the content of Paeonolum in Fufang Yuanhu Zhitong Pian by HPLC. Methods Hypersil BDS C_(18) column was used with methyl alcohol-ware (1 : 1) as the mobile phase, detection wavelength as 274 nm, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.103～0.824 ?g for aeonolum, and linear equation was Y= 5 043.774 9X+275.5915, r=0.9997. The average recovery was 98.59% and the related standard deviation (RSD) was 0.68% (n =6). Conclusion This method was simple, accurate and proper, which provide scientific quantitative analysis method of Paeonolum in Fufang Yuanhu Zhitong pian and the repeatability was good.
