Determination of Amygdalin in Semen Pruni by HPLC
- VernacularTitle:高效液相色谱法测定郁李仁中苦杏仁苷的含量
- Author:
Ping QIAN
;
Yun JIA
;
Zhihui LIU
- Publication Type:Journal Article
- Keywords:
Semen Pruni;
amygdalin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of amygdalin in Semen Pruni. Methods The analytical column was Supelco ODS Cis column {4.6 mm ? 250 mm, 5 ?m), with methanol-water (20 : 80) as mobile phase. The flow rate was 1.0 mL/min, and the detection wavelength was 210 nm. Results The linear range for amygdalin was 0.06613～4.232 ?g (r=1.0000). The average recovery was 95.91% (RSD=2.29%). Conclusion The method is accurate and reliable, and can be used in the determination of amygdalin in Semen Pruni.
