Study on the Transfer Rate of Alkaloids in Extraction and Purification Process of Cortex Phellodendri Amurensis
- VernacularTitle:关黄柏提取纯化过程中生物碱转移率的考察
- Author:
Guoliu CHEN
;
Donghui LIU
;
Qiongdan ZHANG
;
Haizhen HUANG
;
Biyu WU
;
Gang WEI
- Publication Type:Journal Article
- Keywords:
Cortex Phellodendri Amurensis;
Berberine hydrochloride;
Palmatine hydrochloride;
AB-8 macroporous resin;
Transfer rate
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the transfer rate of alkaloids in extraction and purification process of Cortex Phellodendri Amurensis (CPA) and to ascertain the optimal purification technology.Methods We purified the alkaloids from Cortex Phellodendri Amurensis by AB-8 macroporous resin.With the contents of berberine hydrochloride and palmatine hydrochloride as the reference indexes,content determination of alcohol extract,sample solution and eluent from AB-8 macroporous resin were determined by HPLC.Results The optimal technology was as follows:using 8-fold 75 %(V/V) ethanol as extract,extracting for 3 times and lasting one hour for each time.The transfer rate of alkaloids was 83.75 %under the above condition.The transfer rate of alkaloids was about 80 %when the concentration of sample solution(pH value being 8 and centrifugation) was 0.1 g/mL,and the transfer rate of alkaloids in eluent was 75.14 %.Conclusion The optimum technology is stable and feasible,and the purification effect of alkaloids from Cortex Phellodendri Amurensis by AB-8 macroporus resin is good.
