Effects of Tanshinone ⅡA on Arrhythmia and Levels of Ischemia Modified Albumin and Heart Type Fatty Acid Binding Protein in Rats with Myocardial Ischemia-reperfusion Injury
- VernacularTitle:丹参酮ⅡA对缺血再灌注损伤大鼠心律失常及IMA、H-FABP水平的影响
- Author:
Ping YANG
;
Yuhua JIA
;
Jie LI
- Publication Type:Journal Article
- Keywords:
TanshinoneⅡA;
Ischemia-reperfusion injury;
Arrhythmia;
Iischemia modified albumin (IMA);
Heart type-fatty acid binding protein(H-FABP);
Rats
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of tanshinoneⅡA (TⅡA) on arrhythmia and levels of ischemia modified albumin (IMA) and heart type-fatty acid binding protein (H-FABP) in rats with myocardial ischemia-reperfusion injury.Methods Forty Wistar rats were equally randomized into 5 groups:pseudo-operation group (normal saline),model group (normal saline),and high-,middle-and low-dose TⅡA groups (in the dose of 20,10 and 5 mg?kg-1?d-1 respectively).The rats were treated for 7 days,and received operation 2 hours after the last medication.Rats in pseudo-operation group received threading without ligation,while animal model of myocardial ischemia-reperfusion injury was established by ligating the left-anterior coronary artery for 15 minutes and then loosening for 30 minutes.During the operation,electrocardiogram was traced continuously to observe the arrhythmia.HE staining was used to observe the pathological changes of myocardium.Levels of IMA and H-FABP in myocardium were determined by enzyme-linked immunosorbent assay (ELISA).Results Ischemic changes of the myocardium and arrhythmia occurred,myocardial IMA level increased and H-FABP level decreased in the model rats.TanshinoneⅡA obviously attenuated arrhythmia caused by ischemia-reperfusion injury (P
