Anti-metastasis Activity of Earthworm Fibrinolytic Enzyme on Hepatoma Cell in Vivo
- VernacularTitle:蚯蚓纤溶酶抗肝癌转移作用的实验研究
- Author:
Chunxia CHANG
;
Hong CHEN
;
Hong JI
;
Zhiguo ZHANG
- Publication Type:Journal Article
- Keywords:
Earthworm fibrinolytic enzyme;
Liver cancer;
Metastasis;
Orthotopic transplantation model;
Nude mice
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of earthworm fibrinolytic enzyme (EFE) on the metastasis of human hepatoma cells.Methods A metastatic model of human hepatocellular carcinoma in nude mice via orthotopic implantation was established.After the modeling for 7 days,the mice were divided randomly into the control group,low-dose EFE group (800 uku/kg?d-1),and high-dose EFE group (1600 uku/kg?d-1).After administration for 30 days,the implanted tumors were weighted,and the intrahepatic transmission rate and abdominal cavity seeding rate were calculated after examination by naked eyes.Then the pulmonary metastasis were examined under microscope,and the expression of focus adhesion kinase (FAK) and ?1-intigrin were detected through RT-PCR and western blotting method.Results Compared with the model control group,the mean weight of the orthotopic tumor was reduced,and the intrahepatic transmission rate and abdominal cavity seeding rate were decreased in FEF groups (P
