Determination of three components in Qingfei Huatan Pill by RP-HPLC
- VernacularTitle:RP-HPLC同时测定清肺化痰丸中3种成分含量
- Author:
Yanqin ZHU
;
Wenhui WANG
;
Jun YANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
naringin;
hesperidin;
baicalin;
Qingfei Huatan Pill
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a RP-HPLC for determing the contents of naringin,hesperidin and baicalin in Qingfei Huatan Pill(Arisaema Cumbile,Fructus Aurantii,Pericarpium Citrireticulatae,Radix Scutellariae,etc.). METHODS: Naringin,hesperidin and baicalin were separated on a RP C_(18) column.Acetonitrile-water(20(∶)80)(adjusted to pH 2.2 with phosphoric acid) was used as the mobile phase.The detective wavelength was set at 280 nm. RESULTS: The linear ranges were in 1.24-6.2 ?g for naringin (r=(0.999 7))、1.1-5.5 ?g for hesperidin(r=(0.999 8)) and 2.32-11.6 ?g for baicalin(r=(0.999 9)).The recoveries were 99.29% with RSD of 1.78% for naringin、100.08% with RSD of 1.44% for hesperidin and 98.76% with RSD of 1.42% for baicalin respectively. CONCLUSION: The method is simple,accurate and reproducible.It may well be used for the quality control of this product.
