Fingerprints of saline semen Trigonellae by HPLC
- VernacularTitle:盐胡芦巴HPLC指纹图谱研究
- Author:
Yun YANG
;
Xiaohua XU
;
Fugang LIU
;
Gongsheng XIAO
- Publication Type:Journal Article
- Keywords:
saline semen Trigonellae;
HPLC;
fingerprint
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the HPLC fingerprints of semen Trigonellae processed with salt-water.(METHODS:)HPLC analysis was performed on a phenomenex Hydro-RP 80A(4.6 mm?250 mm,5 ?m) with mobile phase acetonitrile-water under isocratic and gradient elutions,and detection wavelength was 211nm,the flow rate was 1 mL/min,column temperature was 40 ℃. RESULTS: HPLC fingerprint of 17 main peaks was separated.It was found that the preparative HPLC of standardized processing saline semen Trigonellae from different places have no significant differences. CONCLUSION: The method is reliable,accurate and can be used for quality control of saline semen Trigonellae.
