Content Determination of Berberine in Huangju Detoxication Tablets
- VernacularTitle:黄菊解毒片中盐酸小檗碱含量测定
- Author:
Yanhong JIANG
- Publication Type:Journal Article
- Keywords:
Huangju Detoxication Tablets;
berberine;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method to determine the content of berberine in Huangju Detoxication Tablets. Methods HPLC was used with Diarnonnsil C_(18) (4.6 mmr250 mm, 5 lm) as column, acetonitrile-0.033 mol/L potassium dihydrogen phosphate (30 : 70) as mobile phase, detection wavelength was at 265 nm, flow rate was 1.0 mL/min. Results In berberine range of 0.0432～0.864 ?g, the escope and content of the sample peak area had a good linear relationship, reproducibility of RSD=0.89% (n =6), and the average recovery rate was 99.64% and RSD=0.74% (n =5). Conclusion This method is stable, reliable, with high sensitivity and good reproducibility, and can be used for quality control of Huangju Detoxication Tablets.
