Content Determination of Sinomenine Hydrochloride in Sinomenine Hydrochloride External Applied Powder by HPLC
- VernacularTitle:HPLC法测定盐酸青藤外敷散中盐酸青藤碱含量
- Author:
Yuyan GUO
;
Min LI
;
Lei SHI
- Publication Type:Journal Article
- Keywords:
Sinomenine Hydrochloride external applied powder;
Sinomenine Hydrochloride;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of content in Sinomenine Hydrochloride external applied powder. Methods Diamonsil ODS C_(18) (250 mm ? 4.6 mm, 5 ?m) column was used in HPLC with mobile phase of phosphate buffer (0.01 mol/L K_2HPO_4 solution, and 1%0 triethylamine, pH was adjusted to 3.0)-MeOH (80 : 20). The flow rate was 1.0 mL/min and the detection wavelength was 265 rim. Results The linear range of Sinomenine Hydrochloride was 0.446～4.46 ?g and the regression equation was Y=9.01 ? 10~6X+102803 (r=0.9999). The average recovery was 99.2%, RSD=0.68% (n =9). Conclusion This method is rapid and accurate with good reproducibility. It can be applied to control the quality of Sinomenine Hydrochloride external applied powder.
