Determination of Chlorogenic Acid,Liquiritigenin,Cinnamic Acid,and Monoammonium Glycyrrhetate in Tong Saimai Pellets by RP-HPLC
- VernacularTitle:高效液相色谱法测定通塞脉微丸中绿原酸、甘草素、肉桂酸和甘草酸单铵盐的含量
- Author:
Weidong LI
;
Baochang CAI
;
Liuqing DI
- Publication Type:Journal Article
- Keywords:
TONG SAIMAI PELLETS/chemistry;
CHLOROGENIC ACID/analysis;
CINNAMIC ACID/analysis;
LIQUIRITIGENIN/analysis;
MONOAMMONIUM GLYCYRRHETATE/analysis;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the simultaneous determination of chlorogenic acid,liquiritigenin,cinnamic acid,and monoammonium glycyrrhetate in Tong Saimai Pellets.Methods A Zorbax 80A Extend-C18 column was used.The mobile phase consisted of acetonitrile and 0.1% phosphoric acid,and was used for gradient elution.Chlorogenic acid,liquiritigenin,cinnamic acid and monoammonium glycyrrhetate were determined by dual wavelength,?s=276nm,?R=590nm.Results The linear range of chlorogenic acid was 0.0505~1.6160 ?g,and r=0.9999;the average recovery was 98.17%,and sR=4.28%(N=6).The linear range of liquiritigenin was 0.0252~0.8064 ?g,and r=0.9999;the average recovery was 93.76%,and sR=1.94%(N=6).The linear range of cinnamic acid was 0.0111~0.3552?g,and r=0.9999;the average recovery was 98.25%,and sR =2.72%(N=6).The linear range of monoammonium glycyrrhetate was 0.3300~10.5600?g,and r=0.9999;the average recovery was 102.6%,and sR=1.93%(N=6).Conclusion This method is convenient,rapid and accurate,and it can be used for quality control of the production of Tong Saimai Pellets.
