Inhibitory Effect of Human Serum Containing Niaoduqing Capsule on Proliferation and Transdifferentiation of Human Renal Tubular Epithelial Cells
- VernacularTitle:尿毒清胶囊人含药血清抑制尿毒症毒素血清刺激的肾小管上皮细胞-2增殖与转分化作用观察
- Author:
Qiulin ZHANG
;
Shuifu TANG
;
Qinguo HONG
- Publication Type:Journal Article
- Keywords:
NIAODUQING CAPSULE/pharmacology;
RENAL FIBROSIS/TCD therapy;
SERUM PHARMACOLOGY;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of human serum containing Niaoduqing Capsule(NC)on proliferation and transdifferentiation of human renal tubular epithelial cells(HK-2).Methods The in-vitro cultured HK-2 were divided into 6 groups according to the culture media: normal control group,uremic serum groups(at the concentration of 5% and 10%), NC-containing serum groups(at the concentration of 5% and 10%),and Monopril group(at the concentration of 10-6mmol/L).Cell proliferation was measured by methylene blue assay.Cell life cycle and the percentage of cells with ?-smooth muscle actin(?-SMA)expression positive in every group were evaluated by flow cytometry.Results The optical absorption value in uremic serum groups was higher than that in the normal control group(P
