Simultaneous determination of notoginsenoside R_1,ginsenoside Rg_1,ginsenoside Re,ginsenoside Rb_1 in Yunnan Baiyao Powder by RP-HPLC
- VernacularTitle:RP-HPLC同时测定云南白药中三七皂苷R_1及人参皂苷Rg_1、Re、Rb_1含量
- Author:
Min YANG
- Publication Type:Journal Article
- Keywords:
Yunnan Baiyao Powder;
notoginsenoside R_1;
ginsenoside Rg_1;
ginsenoside Re;
ginsenoside Rb_1;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC method for determing notoginsenoside R_1,ginsenoside Rg_1,ginsenoside Re,ginsenoside Rb_1 in Yunnan Baiyao Powder. METHODS: A RP-HPLC method was applied with a LiChrospher C_(18) column(4.6 mm?250 mm,5 ?m) by gradient elution of acetonitrile-0.4% phosphoric acid solution as the mobile phase.The flow rate was 1 mL/min and the detective wavelength was set at 203 nm.The column temperature was at 30 ℃. RESULTS: All of the four compounds showed good linearities and the recoveries were in the range of 99.6%-101.2%. CONCLUSION: The method is accurate and suitable for the quality control of the product.
