Study on cellulose enzyme extraction technology of Radix Aconiti
- VernacularTitle:川乌的纤维素酶酶解提取工艺研究
- Author:
Yongsheng LI
;
Qiang LIN
;
Laili ZHENG
;
Pingfang TIAN
- Publication Type:Journal Article
- Keywords:
Radix Aconiti;
cellulose enzyme;
aconitine;
aconitum alkaloids;
orthogonal design
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the extraction methods of cellulose enzyme,semi-bionic and alcohol reflux for the impact of aconitine and aconitum alkaloids yields,and to optimize the extraction method. Methods The optimum condition of enzymatic extraction has been obtained by orthogonal test and compared with semi-bionic and alcohol reflux extraction by taking the contents of aconitine and aconitum alkaloids as indexes.Results The optimum extraction conditions were as follows:the temperature was 45℃, pH value was 4.5,the amount of cellulose enzyme was 8 mg/g,the extracting time was 5 h and the extraction rate of aconitine was 0.002 447%,aconitum alkaloids was 0.244 410%.The extraction rates of aconitine and aconitum alkaloids of semi-bionic extraction were 0.001 735%and 0.189 340%,respectively. The extraction rates of aconitine and aconitum alkaloids of alcohol reflux extraction were 0.001 869% and 0.200 720%respectively.The enzymatic extraction has a significant advantage.Conclusion The enzymatic extraction significantly improve the extraction rates of aconitine and aconitum alkaloids,it could be applied in practice.
