Solanine on apoptosis by regulating glutathine hormone and reactive oxygen species to damage mitochondrial structure in HepG2 cell
- VernacularTitle:龙葵碱调控还原型谷胱甘肽和活性氧氧化还原体系损伤线粒体超微结构诱导HepG2细胞凋亡
- Author:
Shiyong GAO
- Publication Type:Journal Article
- Keywords:
solanine;
apoptosis;
mitochondria;
reactive oxygen species(ROS);
reduced glulathione hormone(GSH)
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between apoptosis and mitochondria damage.Methods The cell morphology was observed under inverted microscope.Staining with Annexin V/PI,the cell apoptosis was observed by laser confocal scan microscope and the apoptosis rate was analyzed by flow cytomety. Transmission electron microscope(TEM) was used to observe mitochondrial structure.ROS was determined by confocal.Colorimetry assay was adopted to determine the reduced glutathione hormone (GSH).Results The cell number in solanine groups was fewer than that in control group,and some died.Staining with Annexin V/PI,Annexin V-FITC was high fluorescent in solanine groups,which is obviously apoptosis features.The early apoptosis rate is 4.0%,8.5%,and 20.1%inducing by 0.4,2,and 10 mol/L solanine for 24 h,respectively.Swelling mitochondria,absence of mitochondria crests and vesicles were found in mitochondria of HepG2 cell treated by solanine for 24 h.The ROS levels increased and GSH level decreased in solanine groups.Conclusion Solanine could induce the apoptosis and the effect may be attributed to decrease GSH,which interrupts the immediate remove of ROS so as to damage the mitochondria.
