shRNA-mediated interference of gene expression PLC? downturn on T24 bladder cancer cells
- VernacularTitle:shRNA干扰介导PLC?基因表达下调对膀胱癌T24细胞生长的影响
- Author:
Chunli LUO
- Publication Type:Journal Article
- Keywords:
Bladder carcinoma;
PLC?G gene;
RNA interference;
Cell proliferation;
Proliferating cell nuclear antigen
- From:
Journal of Chongqing Medical University
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether PLC? gene down regulation by RNA interference(RNAi) leads to inhibition of proliferation in human bladder carcinoma T24 cell.Methods: The shRNA recombinant plasmids targeting to PLC? gene was constructed and transfected into bladder carcinoma T24 cell with Lipofectamine 2000.RT-PCR was used to monitor the validity of specific s h R N A in down regulation of PLC?.Then MTT assay was performed for detecting cell proliferation,the changes of PCNA were analyzed by immunocytochemical method,Electron microscope was used to observe the morphological changes.Results: The specific PLC? shRNA was confirmed to be efficient in silencing PLC? expression.PLC? gene down regulation by the shRNA recombinant plasmids inhibition cell proliferation rate about 78.01%,They were significantly different from that of control group(P
