Simultaneous determination of danshensu,protocatechuic aldehyde,caffeic acid,rosmarinci acid,salvianolic acid B,and salvianolic acid A in Xiangdan Injection by HPLC
- VernacularTitle:HPLC法测定香丹注射液中丹参素、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸B和丹酚酸A(英文)
- Author:
Jicheng YUAN
;
Yongqin YIN
;
Zhibin SHEN
- Publication Type:Journal Article
- Keywords:
Xiangdan (XD) Injection;
water-soluble chemical constituents;
HPLC
- From:
Chinese Traditional and Herbal Drugs
2009;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method to determine simultaneously the content of six water-soluble chemical constituents (danshensu,protocatechuic aldehyde,caffeic acid,rosmarinci acid,salvianolic acid B,and salvianolic acid A) in Xiangdan Injection,and to compare their content discrepancy of Xiangdan Injection from 20 manufacturers.Methods The HPLC method was established.The column was Alltima C18 (250 mm?4.6 mm,5 ?m) at the gradient elution mode with the flow rate of 1.0 mL/min,column temperature was kept at 25 ℃ and the detector was set at 288 nm.Results Six components were separated clearly.The relationship between the concentration and the peak areas of the six compounds was all linear.The precision,stability,repetition,and average recovery were complied with the limit.Significant difference was found in contents of six chemical constituents in Xiangdan Injections from different manufacturers that the variation of salvianolic acid A content was the most distinct and danshensu was the most quietly.Conclusion The method has been successfully used to quanlify the six compounds in Xiangdan Injection,and then,can offer the reference for the quality control of Xiangdan Injections roundly.
