Inhibitory Effects of Circadian Gene Per2 on Human Pulmonary Carcinoma Cell
- VernacularTitle:近日节律基因Per2对人肺癌细胞生长的抑制作用
- Author:
Jun RUI
;
Hui HUA
;
Yanyou LIU
- Publication Type:Journal Article
- Keywords:
circadian gene;
carcinoma;
apoptosis;
pulmonary carcinoma
- From:Space Medicine & Medical Engineering
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the effects of circadian gene Per2 on growth of human lung cancer cell A549 cell and its mechanism. Methods pcDNA3.1(+)-Per2 was transfected into A549 cells and controlled with the vector pcDNA3.1(+). The expression of Per2 was confirmed with RT-PCR and western blot. The Inhibitory effect of Per2 on cell growth and proliferation was demonstrated with MTT assay and colony-forming assay. Apoptosis was detected with flow cytometry. Results The circadian gene Per2 exhibited a growth-inhibitory and apoptosis-inductory effect on A549 cells. Conclusion The results indicate that Per2 may inhibit A549 cell proliferation,which mechanism might involve cellular apoptosis.
