Determination of Chlorogenic Acid and Hyperoside in Leaves of Acanthopanax Senticosus Produced in Different Areas by HPLC
- VernacularTitle:HPLC法测定不同产地刺五加叶中绿原酸和金丝桃苷含量
- Author:
Jing SHAO
;
Liuqing DI
;
Xin XIE
- Publication Type:Journal Article
- Keywords:
the leaves of acanthopanax senticosus;
Chlorogenic acid;
Hyperoside;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the content determination of Chlorogenic acid and Hyperoside in the leaves of acanthopanax senticosus from different areas.Methods The samples were separated on Hedera? ODS-2 C18 column,using acetonitrile-0.4% phosphoric acid(17∶83) as mobile phase,the detection wavelength was 360 nm.Result Chlorogenic acid and Hyperoside showed good linear relationship over the ranges of 10.2～306 ?g/mL(r =0.999 5) and 10.4～312 ?g/mL(r =0.999 8),respectively.The average recoveries were 97.14% and 98.21% with RSD at 1.65% and 1.09%,respectively.The contents of Chlorogenic acid and Hyperoside were different obviously in samples from different areas.Conclusion This method is simple,rapid and sensitive,and it can be used for the further development and the quality control of the leaves of acanthopanax senticosus.
