Content Determination of Emodin and Chrysophanol in Cigu Xiaozhi Pill
- VernacularTitle:慈菇消脂丸中大黄素和大黄酚含量的测定
- Author:
Ziming JIN
;
Jinhui WANG
;
Shaojun YANG
- Publication Type:Journal Article
- Keywords:
Cigu Xiaozhi pill;
emodin;
chrysophanol;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of the content of emodin and chrysophanol in Cigu Xiaozhi pill.Methods The chromatographic procedure was carried out in Symmetry C18(4.6 mm?150 mm,5.0 ?m) column with methanol-0.1% phosphoric acid(85∶15) as the mobile phase,flow rate was 0.8 mL/min,column temperature was 20 ?C,and the detection wavelength was 423 nm.Results Emodin and chrysophanol were well separated with other components.The standard curves of emodin and chrysophanol showed linearity in the range of 0.25～0.75 ?g.The average recoveries were 101.35%(RSD=2.26%) and 98.66%(RSD=1.74%) respectively.Conclusion The method is sensitive,simple and accurate,and can be used for the determination of emodin and chrysophanol in Cigu Xiaozhi pill.
