ISSR Analysis for genetic diversity of Coptis chinensis
- VernacularTitle:黄连遗传多样性的ISSR分析
- Author:
Chunping ZHANG
;
Ping HE
;
Shijun HU
;
Ruibo WANG
;
Yifeng ZHANG
;
Changkun LIU
;
Shan GAO
- Publication Type:Journal Article
- Keywords:
Coptis chinensis Franch.;
genetic diversity;
ISSR;
cluster analysis;
genetic variation
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the genetic diversity of Coptis chinensis.Methods The genetic diversity of 78 individuals from seven populations was analyzed by inter-simple sequence repeat(ISSR).Results Twelve primers were selected to produce highly reproducible ISSR bands.Among 106 amplified bands,72 showed polymorphism,the percentage of polymorphic bands reached to 67.92%.Nei's gene diversity index(H)was 0.180 3,Shannon information index(I)was 0.283 2,Gst was 0.681 5.The genetic distance coefficient and genetic similarity were 0.089 4—0.184 6 and 0.832 1—0.912 7,respectively.ConclusionC.chinensis holds high genetic diversity and the majority of genetic variation occurs among the populations.By cluster analysis,the geographical distribution is very obvious.The ISSR marker could be used for the analysis of the genetic diversity and genetic variation of C.chinensis.
