Effect of survivin expression on the maturation of mouse marrow dendritic cells
- VernacularTitle:Survivin表达对鼠髓样树突状细胞成熟的影响
- Author:
Yanmin LI
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Survivin;
Differentiate
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of survivin expression on the mature process of dendritic cells from mouse bone marrow. Methods:The cells in marrow as the precurosor of the dendritic cells were taken from mouse in bioclean condition. Then the iDC were induced by the precurosor of DCs with GM-CSF and IL-4 in medium. ThemDC were induced by the precurosor of DCs with GM-CSF,IL-4,and TNF-? in medium. Survivin was compared by RT-PCR,and Western-bolt among these three stages. Results:The RT-PCR showed that the expression ratios of survivin mRNA among the precurosor of DCs,iDC,and mDC were(0.735?0.081),(0.596?0.073,P
