Effects of autologous bone marrow mesenchymal stem cells on the renal cell proliferation and apoptosis and renal function in a rabbit of renal ischemia-reperfusion injury
- VernacularTitle:兔自体骨髓间充质干细胞移植对冷缺血再灌注损伤肾脏细胞增殖与凋亡和肾功能的影响
- Author:
Zhenqiang FANG
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Kidney;
Ischemia-reperfusion injury;
Transplantation
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the protective effects of autologous bone marrow mesenchymal stem cells(BM-MSCs) on renal cell proliferation apoptosis during cold ischemia-reperfusion injury(IRI) in rabbit and to probe the mechanism of protective action on kidney. Methods:45 rabbits were divided randomly into three groups(n=15): Sham operation group( group Ⅰ), control group of IRI( group Ⅱ and BM-MSCs transplantation group(group Ⅲ). After isolating the BM-MSCs of rabbits in group Ⅲ, to establish the rabbit models of renal IRI. Autologous BM-MSCs were infuded into rabbit in group Ⅲ, and the equivalent physiological saline in rabbit in group Ⅰ and Ⅱ. The detection was taken at the timepoints of 3,7 and 14 days after renal ischemia-reperfusion respectively. The serum levels of Crea and Urea were detected to evaluate the renal function, and Bax, Bcl-2 and PCNA in kidney were dectected with immunohistochemical staining. The apoptosis of cells was detected with TUNEL method. Results:The serum levels of Crea and Urea in group Ⅲ at different timepoint were lower than those in group Ⅱ significantly(P
