The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats
- VernacularTitle:FasL基因转染诱导大鼠肾移植免疫耐受的实验研究
- Author:
Guangyong XU
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
Gene transfection;
Immunotolerance;
Rat
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Fas ligand(FasL) gene transfection to renal allograft in rats. Methods: Firstly Establish renal transplantation model of rat. Secondly FasL recombinant adenovirus vector was contruccted and transduced into rat renal allografts by renal artery perfusion.Immunohistochemistry were used to detect the expression of exogenous FasI gene. Electronic microscopy was used to observe the changes in the ultrastructure.At the same time,mean survival of animals and the level of serum creatinine were observed.Results:Through animal experiment,we found: the mean survival time was 31.3 days in Ad-FasL treated group, whereas the the mean survival time of without treatment group was 9.1 days. The mean survival time increased 22 days in Ad-FasL treated group.There has significant difference. Conclusions:Adenoviral vector can successfully transfection rat kidneys with the FasL cDNA. FasL gene transfection can protect rat renal allografts from immunologic attack and prolong the allograft surviva1.
