The differential expression of VEGF_(165)b in the transitional cell carcinoma of the bladder and normal bladder tissues and its clinical significance
- VernacularTitle:膀胱移行细胞癌组织与正常组织中VEGF_(165)b的差异表达及其意义
- Author:
Cuiping JIANG
- Publication Type:Journal Article
- Keywords:
Bladder neoplasm;
VEGF165b;
Reverse transcriptase polymerase chain reaction (RT-PCR);
Immunohistochemistry
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differential expression of VEGF165b in the transitional cell carcinoma of the bladder (TCCB) and normal bladder tissues and its role in the development of TCCB. Methods: S-P immunohistochemistry was used to detect the expression of VEGF165b protein in 38 specimens of paraffin-embedded TCCB tissues and 36 specimens of paraffin-embedded normal bladder tissues. RT-PCR was performed to detect the expression of VEGF165b mRNA in 55 specimens of fresh TCCB tissues and 43 specimens of fresh normal bladder tissues. Results: Among 36 of normal bladder tissues, 35 specimens had positive expression of VEGF165b protein, with the positive rate of 97.22% (35/36) that was significantly higher than 21.05% (8/38) (P
