Inhibitory effects of VEGF antisense oligodeoxynucleotides on bladder carcinoma in nude mice mediated by ultrasound microbubble
- VernacularTitle:超声微泡介导VEGF反义寡核苷酸对裸鼠膀胱癌移植瘤生长的抑制作用
- Author:
Yu GAO
- Publication Type:Journal Article
- Keywords:
Ultrasound microbubbles;
VEGF;
Bladder tumor;
Antisense oligonucleotides
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of inhibition of vascular endothelial growth factor(VEGF) antisense oligodeoxynucleotides(ASODN) on human bladder carcinoma in nude mice mediated by ultrasound microbubble. Methods:The mice were randomly divided into 3 groups when transplantation tumor was established: For UM+ASODN group, mixture of microbubble and VEGF-ASODN was inject into every mice from caudal vein plus ultrasound tumor local irradiation. For UM group, microbubble was inject into very mice from caudal vein plus local irradiation with the same condition. For control group, physiological saline was inject into every mice from caudal vein.The treatment was performed every 3 days with total 5 times.The volume change of mouse subcutane tumors was observed.Evaluate the expression of VEGF and CD34 and Ki67 with immunohistochemistry dyeing and calculate the microvessel density (MVD) and cell proliferation index;TUNEL assay was used to analyse the apoptosis of tumor cells. Results:The growth speed of nude mice tumor in UM+ASODN group was obviously slower than control group; The expression of VEGF and The MVD in UM+ ASODN group was lower than control group(P
