The experimental study of bFGF-fibrin gule-amniotic membrane on corneal alkali burns
- VernacularTitle:bFGF胶联羊膜缓释药膜治疗角膜碱烧伤的实验研究
- Author:
Mingming CAI
- Publication Type:Journal Article
- Keywords:
Sustained release;
Fibrin glue;
Amniotic membrane;
Fibroblast growth factor, basic(bFGF);
Alkali burn
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of a new medical biomaterial—bfgf-fibrin glue-amniotic membrane(bFAM) for corneal alkali burn and provide experimental foundation for the clinical application in the future. Methods: We use fibrin glue compounded with bFGF and amniotic membrane to prepare the bFAM. 45 New Zealand rabbits were used to make corneal alkali burn models and divided into 3 groups:bFAM group、amniotic membrane group、comparison group, each group have 15 rabbits. After operation, we observe the linical curative effect. 7d、14d and 28d after operation, 5 corneas were extracted for histopathological analysis. The expression of bFGF in corneas was measured by immunohistochemistry analysis. Results: After operation,bFAM group have better curative effect than amniotic membrane group in Inflammatory reaction、tissue repair and anti-CNV. The expression of bFGF in bFAM group is much higher than amniotic membrane group and comparison group in the day 7 afer operation, and in other time points the expression have not distinct difference in all groups. Conclusion: Compared with single layer amniotic membrane, bFAM shows better curative effect for corneal alkali burn.
