Preliminary proteomic study on protective mechanism of carvedilol pre-treatment for ischemia-reperfusion heart injury in rats
- VernacularTitle:卡维地洛预处理对大鼠缺血再灌注损伤心肌蛋白质组影响的初步研究
- Author:
Zhao GAO
- Publication Type:Journal Article
- Keywords:
Carvedilol;
Myocardial ischemia-reperfusion injury;
Two dimensional gel electrophoresis;
Proteomics;
Rat
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of the protein expression in heart tissue of rats after ischemia-reperfusion(I/R) injury and pre-treatment of carvedilol, and to elucidate the new protective mechanism of carvedilol pre-treatment to I/R heart injury. Methods:18 rats were randomly divided into sham-operation group, I/R injury group and carvedilol pre-treated group. The model of myocardial I/R injury was established by 30min ligation of the left anterior descending branch of the coronary artery and 2h reperfusion. Serum malondiadehyde (MDA) was determined, and the cytosolic fraction of myocardial tissue homogenate was separated applying two-dimensional gel electrophoresis technique. Differentially expressed protein spots were figured out with PDQuest software, while preliminary protein-identification was carried out by means of the two-dimensional gel electrophoresis database on the web. Results:Compared with the sham-operation group, after I/R injury, serum MDA was increased(P
