Determination of the Plasma Protein Binding Rate of Plasma Brucine in Rats by HPLC
- VernacularTitle:高效液相色谱法测定大鼠血浆中马钱子碱与血浆蛋白的结合率
- Author:
Jinhua XU
;
Jun CHEN
;
Baochang CAI
;
Ying GAO
- Publication Type:Journal Article
- Keywords:
Brucine;
Protein binding rate;
Balance dialysis;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein binding rate of brucine in plasma of rats.Methods The plasma balance dialysis was used.HPLC was performed for the quantitative determination of brucine,and then the protein binding rate of brucine in rats plasma was calculated.Results The protein binding rate was 59.679 %,59.935 %,and 56.387 %when the concentration of plasma brucine was 2.5,1,and 0.4 ?g?mL-1 respectivley.Conclusion The HPLC method used to determine brucine is simple,rapid,and sensitive,and with good specificity,precision and accuracy.Brucine has medium capacity in binding to plasma protein.
