HPLC Fingerprint Analysis of Medicinal Slices of Radix et Rhizoma Rhei
- VernacularTitle:大黄饮片HPLC指纹图谱的方法学研究
- Author:
Cuiling LIU
;
Donghui LIU
;
Yuechun HUANG
;
Gang WEI
- Publication Type:Journal Article
- Keywords:
Radix et Rhizoma Rhei;
Fingerprint;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a fingerprint analysis method for the medicinal slices of Radix et Rhizoma Rhei.Methods HPLC with ZORBAX Eclipse XDB-C18 was applied.The chromatographic conditions were as follows:acetonitrile-0.1 %H3PO4 solution(gradient alution) as a mobile phase and detection wavelength at 254 nm,flow rate at 1 mL?min-1,and column temperature being 40 ℃.Results Twenty-five common peaks were separated from 10 batches of medicinal slices of Radix et Rhizoma Rhei.Conclusion The method is reliable and accurate,and can be used as a quality control method for the medicinal material of Radix et Rhizoma Rhei.
