Protection in vitro of aFGF on human liver cell L-02 and human kidney cell 293 caused by nitidine chloride
- VernacularTitle:氯化两面针碱对肝、肾的毒性及酸性成纤维细胞生长因子对其的保护作用研究
- Author:
Min WEI
;
Limin LIU
;
Danni LI
;
Huagang LIU
- Publication Type:Journal Article
- Keywords:
nitidine chloride;
human liver cell L-02;
human kidney cell 293;
toxicity;
acidic fibroblast growth factor
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect the inhibition effect of nitidine chloride on proliferation of human liver cell L-02 and human kidney cell 293 and the protective effect of acidic fibroblast growth factor(aFGF) on human liver cell L-02 and human kidney cell 293 damaged by nitidine chloride in vitro. METHODS: The MTT assay was used to assess the proliferation of human liver cell L-02 and human kidney cell 293 treated with nitidine chloride.The contents of SOD and MDA and LDH in cultural supernate were measured by ultraviolet spectrophotometry. RESULTS: Nitidine chloride inhibited the proliferation of human liver cell L-02 and human kidney cell 293 in a dose-dependent manner. CONCLUSION: Nitidine chloride has certain toxicity on human liver cell L-02 and human kidney cell 293.aFGF could protect human liver cell L-02 and human kidney cell 293 damaged by nitidine chloride.
