Effect of Cooling Blood and Invigorating Circulation Method on Content of Hydroxyproline and TGF-?_1 in Pulmonary Fibrosis Mice
- VernacularTitle:凉血活血法对肺纤维化小鼠羟脯氨酸和转化生长因子-?_1含量的影响
- Author:
Jing HU
;
Yongjie GUO
;
Xuling LIU
- Publication Type:Journal Article
- Keywords:
pulmonary fibrosis;
TGF-?1;
hydroxyproline;
cooling blood and invigorating circulation method;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To study the effect of cooling blood and invigorating circulation method on prevention and treatment of pulmonary fibrosis,and explore its mechanism.Methods Male ICR mice were randomly divided into 4 groups:negative control group,pulmonary fibrosis model group,cooling blood and invigorating circulation group and prednisone positive control group.The pulmonary fibrosis model of mice were established by BLM nasal instillation.After 8 hours,the mice were given prescription of cooling blood and invigorating circulation(Xijiao Dihuang decoction) by gastric perfusion.At the 28th day after treatment,the indexes were detected.The TGF-?1 content of bronchoalveolar lavage fluid(BALF) was tested by enzyme linked immunosorbent assay,and hydroxyproline(HYP) was examined by alkaline hydrolysis.Results The degree of airsacculitis and fibrosis in model group are obviously higher than the negative control group by pathological observation.Compared with model group,HYP content was decreased obviously in herb group(P
