Determination of Flavonoids in Rubus multibracteatus Levl.et Vant.
- VernacularTitle:大乌泡叶中总黄酮含量测定
- Author:
Wei CHENG
;
Wenjie QIN
;
Suhong CHEN
- Publication Type:Journal Article
- Keywords:
Rubus multibracteatus Levl.et Vant.;
total flavonoids;
content determination;
spectrophotometry
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To supply scientific basis for the reasonable development and utilization of Rubus multibracteatus Levl.et Vant.resources.Method The total flavonoids was extracted from Rubus multibracteatus Levl.et Vant.with 60% methanol and its content was determined by spectrophotometry.The reliability and repeatability of the method were tested through stability,repeatability,accuracy and recovery experiments.Result Both standard and tested solutions had the maximum absorbance at 510 nm.The RSD values of repeatability,accuracy and recovery experiments were 2.11%,0.97% and 2.4% respectively.The results of content determination showed that the total flavonoids in Rubus multibracteatus Levl.et Vant.was 3.8%.Conclusion Extracted with 60% methanol,colored with aluminiumnitrate and determined by spectrophotometry at 510 nm were suitable for the determination of total flavonoids in Rubus multibracteatus Levl.et Vant..
