Content Determination of Quercetin in Pyrola calliatha H.Andres by HPLC
- VernacularTitle:HPLC法测定鹿蹄草中槲皮素的含量
- Author:
Wen ZHANG
;
Yuling LI
;
Libin FENG
- Publication Type:Journal Article
- Keywords:
Pyrola calliatha H.Andres;
quercetin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a method for determining the quercetin in Pyrola calliatha H.Andres.Method HPLC method was carried on Agilent Eclipse XDB-C18 column(4.6 mm?150 mm,5 ?m) with mobile phase of methanol-0.1% phosphoric acid(50:50),and the detecting wavelength was at 370 nm.Result The standard curve was linear within the range of 0.092～0.46 ?g.The average recovery rate was 99.24% and RSD was 0.70%.Conclusion The method is simple,accurate,highly sensitive and reproducible.It can be used for the quality determination of quercetin in Pyrola calliatha H.Andres.
