Combined effects of hyperbaric oxygen and mannitol on the apoptosis and ultramicrostructure changes of cells of limb ischemia and reperfusion injury of different phases in rats
- VernacularTitle:高压氧与甘露醇联合作用对不同时相肢体缺血再灌注损伤细胞凋亡与组织超微结构的影响
- Author:
Bo GAO
- Publication Type:Journal Article
- Keywords:
Ischemia-reperfusion injury;
Apoptosis;
Hyperbaric oxygen
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the occurrence and pathological changes of the limb ischemia-reperfusion injury of different phases and the combined effects of hyperbaric oxygen and mannitol on the apoptosis and ultramicrostructure changes of rats.Methods:204 SD rats were randomly divided into various groups,underwent different phases of reperfusion after 8 hours of sustained ischemia,and treated with hyperbaric oxygen and/or mannitol for five days.The expression of cell poptosis-related gene Bax,and Bcl-2 was detected through the paraffin sections of rat skeletal muscle.Electron microscope was used to observe the ultramicrostructure changes of the gastrocnemius muscle under the effects of different interventions.Results:In all the intervention groups the Bax levels at 4 h,24 h,72 h after reperfusion were significantly less than in the control groups.In HBO and mannitol-HBO groups the Bcl-2 levels at 4 h,24 h were significantly higher than in the control groups.And in mannitol groups the Bcl-2 levels at 4h were significantly higher.In hyperbaric oxygen groups,the cellular swelling of skeletal muscle and the local cytokine response were significantly lower,almost without microvascular thrombosis in capillary vessel.The sarcomere was rarely collapsed,little plasma membrane was shattered,little mitochondrial swelled or changed into the myelinfigure.The combined effects of hyperbaric oxygen and mannitol were significantly better than single-use of hyperbaric oxygen or mannitol(P
