The influence of centrifugal speed and time on TEM sample preparation of culture cell
- VernacularTitle:离心速度及时间对培养细胞透射电镜样品制备的影响
- Author:
Jingchuan FAN
- Publication Type:Journal Article
- Keywords:
Cultured cells;
Digestion centrifuge;
Sample preparation;
Ultrastructure;
Electron microscope
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influences of different centrifugal conditions on the ultrastructure of cultured cells in the digestion centrifugation to find out the best speed and time of centrifugation.Methods:The cultured cells after digested,were divided into groups according to different digestion centrifuge speed and time,and the changes of cell morphology in each group at different centrifugation time and speed were compared under transmission electron microscope.Results:Enough cells could mot be collected with the centrifuge speed less than 800 r/min,8 min.The cells were deformed due to extrusion or the cytoplasm was torn with the speed of 2 000 r/min,15 min.With the speed of 800～1 500 r/min,8～15 min,it was more proper to prepare samples and preserve cell ultrastructure.Conclusion:It is ideal to prepare TEM samples of cultured cells by using the cells being digested and centrifuged with speed at 800 ~1 500 r/min and time of 8~15 minutes.
