Expression and postmortem stability of HSP70 in hippocampus of hemorrhagic shock rat
- VernacularTitle:失血性休克大鼠海马中HSP70表达及死后稳定性研究
- Author:
Junhua CAO
- Publication Type:Journal Article
- Keywords:
Immunohistochemistry;
Heat-shock proteins;
Hemorrhagic shock;
Postmortem stability
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and postmortem stability of HSP70 in hippocampus of hemorrhagic shock rat.Methods:105 healthy SD rats were randomly divided into 5 groups:normal group,sham operation group,hemorrhagic shock group,traumatic control group and postmortem stability group.In hemorrhagic shock group and postmortem stability group,rats were submitted by controlled bleeding,with the mean arterial pressure kept around 40mmHg.And in traumatic group,brain contusion was induced by piston impact.After that,the regulation of HSP70 expression in hippocampus of each group was detected by immunohistochemistry technique,and evaluated with image analysis technique and statistic system.Results:One hour after hemorrhagic shock,HSP70 expression in hippocampus was significantly higher in each shock subgroups compared with that in normal group and sham group(P0.05).Conclusion:HSP70 is specifically expressed in hippocampus of hemorrhagic shock rat,and showed nice postmortem stability under the environment of -20℃.
