Content determination of trace kryptofix 222 in ~(18)F-fluorodeoxyglucose
- VernacularTitle:~(18)F-氟脱氧葡萄糖中微量氨基聚醚(2.2.2)含量测定
- Author:
Qiming LI
- Publication Type:Journal Article
- Keywords:
18F-Fluorodeoxyglucose;
Kryptofix 222;
Ultraviolet Spectrophotometry;
Content determination
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an ultraviolet spectrophotometry method for determination of trace kryptofix 2.2.2 in 18F-fluorodeoxyglucose product.Method:A storage solution was prepared by adding properly benzaldehyde and lead nitrate to citric acid-NaOH buffer solution(pH = 6.4),and ultralviolet absorbance curve of the solution was changed to platform at the wavelength of 250 nm nearby,and the absorbance value of this solution was 0.4Abs ~ 0.6Abs at 250 nm.Properly 18F-fluorodeoxyglucose product was added to storage solution,and the absorbance value of trace Pb(Ⅱ)-kryptofix 222 complex was detected by ultraviolet Spectrometry for quantitative determination of trace kryptofix 222.Results:The absorbance coefficient of complex was 1.6?104 L/(mol?cm) at 250 nm;the linear range of kryptofix 222 was 0.0?g/ml~10.0?g/ml,the limit ofkryptofix 222 was 0.0?g/ml~10.0?g/ml,the limit of quantification was 0.13?g/ml;the adding recovery was 97.2%～102.7%,with the average recovery of 99.7%;the relative standard deviation(RSD) was 2.4%(n=5).Conclusion:The method was specific,sensitive and suitable for determination of trace kryptofix 2.2.2 in 18F-fluorodeoxyglucose product.
