Mechanisms of magnolol against 6-OHDA-induced PC12 cell trauma
- VernacularTitle:厚朴酚对抗6-OHDA诱导PC12细胞损伤的作用机理研究
- Author:
Xiyong YE
;
Hong ZHAO
;
Changhe CHENG
;
Yuren ZHANG
- Publication Type:Journal Article
- Keywords:
magnolol;
PC12 cell;
6-OHDA;
Parkinson's disease
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the possible mechanism of magnolol on the damage of PC12 cell induced by the addition of 6-OHDA placed in an incubator for 24 h with rat's pheochromocytoma cell of adrenal medulla in the absence and presence of magnolol. METHODS:Cell viability was measured by MTT method. Reactive oxygen species (ROS) and aspartate-specific cysteine proteases-3 (Caspase-3) concentration(s) were assayed by fluorescence spectrophotometer. RESULTS:Cell activity declined sharply by the addition of 6-OHDA at concentration of 100 ?mol/L,the pre-treatment of magnolol(0.1~10 ?mol/L)could significantly increase the PC12 cell activity and prevent the increased ROS and caspase-3. CONCLUSION:Magnolol may protect the cell trauma induced by 6-OHDA,and the mechanism is related to inhibiting the 6-OHDA-induced elevation in intracellular ROS levels and reducing the caspase-3 activity.