Determination of epimedin C and icariin in Xianling Guobao Capsules by RP-HPLC
- VernacularTitle:HPLC测定仙灵骨葆胶囊中淫羊藿苷和淫羊藿定C含量
- Author:
Qing WU
;
Ning ZHOU
;
Zexi FENG
;
Yong HE
;
Ye LIN
- Publication Type:Journal Article
- Keywords:
Xianling Guobao Capsules;
epimedin C;
icariin;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To set up a method for determining epimedin C and icariin in Xianling Guobao Capsules(Herba Epimedii,Radix et Rhizoma Salviae miltiorrhizae,Fructus Psoraleae,Radix Rehmanniae,etc.). METHODS:The chromatographic conditions included the column of Spherisorb C 18 (4.6 mm?250 mm,5 ?m),the mobile phase was acetonitrice and water as gradient eluent was at a flow rate of 1.0 mL/min,the detection wavelength was set at 270 nm and the column temperature was at 25 ℃. RESULTS:The linear range of epimedin C was 0.22-2.20 ?g and icariin was 0.04-0.40 ?g,respectively. The average recovery of epimedin C and icariin were 103.2% (RSD=3.1%) and 97.8% (RSD=3.2%),respectively. CONCLUSION:The method is reliable,stable and well reproducible,and can control the quality of Xianling Guobao Capsules.
