Purification of Polysaccharide from Angelica sinensis by Ultrafiltration Membrane Technology
- VernacularTitle:超滤膜纯化当归多糖的研究
- Author:
Qin FAN
;
Yingdong LI
;
Wenjun ZHAO
- Publication Type:Journal Article
- Keywords:
Angelica sinensis polysaccharide;
ultrafiltration;
purification;
orthogonal test
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and purify polysaccharide from Angelica sinensis by ultrafiltration technology. Methods Extract of polysaccharide of Angelica sinensis was ultrafiltrated with different aperture membrane, the separation parameters on membrane separation efficiency of polysaccharide of Angelica sinensis were optimized by and orthogonal test. Results Polysaccharide of Angelica sinensis was separated with 200, 100, 50, 20 kD membrane. The contents of polysaccharide was 30.47% before ultrafiltration. The main component of Angelica sinensis polysaccharide was the fraction with 200 kD (65.42%). The optimum condition of ultrafiltration was obtained as follow:one times of extract volume, 35 ℃, 0.3 MPa. Conclusion The technology was simple and available, and the content of Angelica sinensis polysaccharide was higher.