Simultaneous Determination of Glycyrrhizin, Liquiritin and Isoliquritigenin in Licorice by HPLC
- VernacularTitle:高效液相色谱法同时测定甘草中甘草酸、甘草苷、异甘草素的含量
- Author:
Yunhua CHEN
;
Xiaoxia ZHAO
;
Wenquan WANG
- Publication Type:Journal Article
- Keywords:
Licorice;
glycyrrhizin;
liquiritin;
isoliquritigenin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC quantitatively test method of glycyrrhizin, liquiritin and isoliquritigenin simultaneously for licorice. Methods The 67% methanol extracts of licorice powder were analyzed by reverse-phase HPLC on an ODS column, using a gradient elution with a mixture of 1% H3PO4 and acetonitrile. The eluate was monitored by UV absorption at 248, 276, 360 and 370 nm. Results The linear equation of glycyrrhizin was Y =1?106X +16 220, r 2=1. The linear equation of liquiritin was Y= 2?106X+49 444, r 2=0.999 5. The linear equation of isoliquritigenin was Y =1?107X +4.466 7, r 2=1. The recovery rate of glycyrrhizin, liquiritin and isoliquritigenin was 98.01%, 102.63% and 98.18% respectively. Conclusion The method is accurate, stable amd reliable, and can be used to determine the three main chemical contents simultaneously.
