Expression of acid-sensing ion channels in rat articular cartilage with adjuvant arthritis
- VernacularTitle:酸敏感离子通道在大鼠佐剂性关节炎关节软骨中的表达
- Author:
Fenglai YUAN
;
Feihu CHEN
;
Xueying HUANG
;
Xia LI
;
Fanrong WU
;
Jingjing RUAN
;
Jun LI
- Publication Type:Journal Article
- Keywords:
Arthritis,experimental;
Ion channels;
Cartilage,articular;
Reverse transcriptase polymerase chain reaction;
Blotting western
- From:
Chinese Journal of Rheumatology
2008;12(5):321-324,插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and significance of acid-sensing ion channels(ASICs)in rat articular cartilage with adjuvant arthritis. Methods Complete Freund's adjuvant(CFA) was prepared by suspending heat-killed Bacillus Calmette Guerin(BCG) in liquid paraffin at 10 mg/ml. CFA-induced arthritis was developed by injection of 100 μl CFA emulsion intradermally into the right hind paw. The morphological changes of articular tissues was observed by light microscope; RT-PCR and immunoblotting analyses were used to detect ASICs in rat articular cartilage with adjuvant arthritis. Results RT-PCR and western blot showed that ASIC1a, ASIC2a and ASIC3 were present in the articular cartilage of normal and model group, the ASICs mRNA levels in the model group were higher than in the normal group detected by semiquantitative analysis (P＜0.01), ASICs protein levels in model group were higher than those in the normal group (P＜0.01) when examined by immunoblotting. Conclusion The results show that the expression of ASICs in AA articular cartilage is enhanced and it may be related with articular cartilage breakdown.
