Effects of four Chinese herb extracts on cell proliferation in IEC-6 cells of rats and their glucose absorption
- VernacularTitle:4种中药提取物对大鼠小肠上皮细胞IEC-6增殖及其葡萄糖吸收的影响
- Author:
Xiaozhen SONG
;
Xiujiang YANG
;
Tian WANG
;
Fenghua LIU
- Publication Type:Journal Article
- Keywords:
Herba Agastaches;
Rhizoma Atractylodis;
Cortax Phellodendri;
Gypsum Fibrosum;
intestinal epithelial cell line(IEC-6 cells);
cell proliferation;
glucose obsorption;
Na+,K+-ATPase
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Chinese herb extracts on cell proliferation and glucose absorption in intestinal epithelial cell line(IEC-6 cells) of rats.Methods The extracts of four Chinese herbs,including Herba Agastaches(HA),Rhizoma Atractylodis(RA), Cortex Phellodendri(CP),and Gypsum Fibrosum(GF),were made.Their suitable concentration on the cell proliferation in IEC-6 cells was determined by MTT method,and glucose absorption and the activity of Na+,K+-ATPase in IEC-6 cells were assayed.A method of real time PCR was applied to the determination of SGLT1 and GLUT2 mRNA expression in the cells.Results Chinese herb extracts treatment altered the cell proliferation in a dose-dependent manner.Moreover,RA volatile oil(50 ?g/mL) and CP alkaloid(10 ?g/mL) treatment increased glucose absorption and the activity of Na+,K+-ATPase genes(P0.05).Conclusion The three extracts treatment,including RA volatile oil,CP aklaloid,and HA volatile oil,could increase glucose absorption and the expression of glucose transport carrier genes,but their regulative mechanism are not totally the same.
