Effect of Safflower Injection on proliferation,apoptosis,and expression of bcl-2/bax gene in hepatic stellate cell in vitro
- VernacularTitle:红花注射液对肝星状细胞HSC-T6增殖、凋亡及凋亡相关基因表达的影响
- Author:
Jun LIU
;
Xuanfu XU
;
Wenjuan YANG
;
Chuanyong GUO
- Publication Type:Journal Article
- Keywords:
Safflower Injection;
hepatic stellate cell(HSC);
proliferation;
apoptosis;
bcl-2/bax
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of Safflower Injection on the proliferation,apoptosis,and expression of bcl-2/bax gene in hepatic stellate cell(HSC) in vitro.Methods HSC Line HSC-T6 was incubated with Safflower Injection at different concertration,cell proliferation was assessed by MTT colorimetric assay.After incubated with Safflower Injection 5,10,and 20 mg/mL,flow cytometry(FCM) was used to detect the content of DNA in HSC-T6.Morphological change of HSC-T6 was observed under microscope and agarose gel electrophoresis for DNA Ladder was used to detect apoptosis.Besides,the early stage of apoptosis was detected with Annexin V-FITC/PI double labbled assay.And real time RT-PCR was used to detect the expression of bcl-2/bax gene.Results The significant inhibition of Safflower Injection on HSC proliferation was observed in a dose-and time-dependent manner.Observed by FCM,the cell ratio in G0/G1 phase with Safflower Injection treatment(10 and 20 mg/mL) for 24 h was increased,which showed the significant difference compared with the control group(P
