Inhibition of procyanidin from Vaccinium vitisidaea on proliferation of cardiac fibroblast in neonatal rats and its mechanism
- VernacularTitle:越橘中原花青素抑制新生大鼠心肌成纤维细胞增殖及其作用机制
- Author:
Yanchun WANG
;
Kuang REN
;
Raosheng GU
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
procyanidin;
cardiac fibroblast(CFb);
angiotensin Ⅱ(AngⅡ)
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of procyanidin from Vaccinium vitisidaea on the proli-feration of cardiac fibroblast(CFb) induced by angiotensin Ⅱ(AngⅡ),and to explore its mechanism.Methods The CFb proliferation of cultured neonatal rat was induced by AngⅡ and detected by MTT assay.The levels of collagen Ⅰ,collagen Ⅲ,and TGF-?1 were measured by ELISA.The change of NO content and iNOS activity were measured by nitric acid reductase method and spectrophotometry.Cell cycle was assessed via flow cytometry(FCM). The expression of cell cycle regulatory protein p27 was determined by the combination of immunocytochemical staining and image analysis software.Results CFb Proliferation,collagen content,and TGF-?1 levels in culture medium were markedly inhibited when CFb were treated with procyanidin at concentration of 25,50,and 100 mg/L(P
