Effects of lipopolysaccharide preconditioning on the activities of nuclear factor kappa-B and the expression of intercellular adhension molecule-1/lymphocyte function associated antigen-1 in rats graft with liver ischemia/reperfusion injury
- VernacularTitle:脂多糖预处理对大鼠肝移植缺血再灌注期肝脏NF-?B活性和ICAM-1/LFA-1分子表达的影响
- Author:
Yang LI
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Reperfusion Injury;
Lipopoly saccharide;
Nuclear factor-? B;
Intercellular adhension molecule -1;
Lymphocyte function associated antigen-1
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects and significances of lipopolysaccharide preconditioning on the activities of NF-?B and the expression of ICAM-1/LFA-1 in rats graft liver ischemia/reperfusion injury. Methods: Male Sprague-Dawley rats were divided into three groups: sham operation group(Sham group),orthotopic liver transplantation group (OLT group) and LPS preconditioning group (LPS group). Only dissecting hepatoduodenal ligament was perfomed in Sham group. Experiments of OLT were performed by two-cuff method in OLT group and LPS group. The activities of NF-?B and the expression of ICAM-1/LFA-1 in hepatic tissue ,the levels of ALT,AST in inferior caval vein blood were detected at 0,60,180 min after dissecting of hepatoduodenal ligament in Sham group and after portal vein reperfusion in OLT group and LPS group. Results: Compared with those in Sham group at the different time points respectively,the activities of NF-?B and the expression of ICAM-1/LFA-1 were higher in OLT group and LPS group(P0.05) at 0 min after reperfusion,they were evidently higher in OLT group than in LPS group (P
