Effect of exogenous S100A6 on human osteosarcoma cell line U2OS proliferation,apoptosis and ?-catenin expression
- VernacularTitle:外源性S100A6对人骨肉瘤细胞株U2OS的增殖、凋亡及?-catenin表达的影响
- Author:
Yinghua CHEN
- Publication Type:Journal Article
- Keywords:
S100A6;
Osteosarcoma;
?-catenin
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: we investigated the biological effects of exogenous S100A6 on osteosarcoma cell line U2OS and explored its possible mechanism. Methods:MTT assay was used to determine S100A6 influence on U2OS cells proliferation and their conce ntration-dependent relationship. The trypan blue exclusion assay was used to observe S100A6 inhibitory effect on U2OS cells proliferation in a time-dependent manner;Hoechst staining was used to determine cell apoptosis;Western blot and immunocytochemical methods to determine expressions of cyto-?-catenin. Results:We found that ,on the first three days,OD values in cells subjected to the recombinant protein S100A6(GST-S100A6)with the concentrations of 30,100,300 and 1 000?g/ml respectively were obviously lower than those in the GST group,by 20.5%,23.7%,32.2% and 36.8% accordingly(P
