The detection of the nucleation shifting process of smad4 signal protein in rat dental papillae cells with quantum dots-smad4 monoclonal antibody fluorescent probes
- VernacularTitle:半导体量子点单克隆抗体荧光探针对牙乳头细胞内Smad4信号蛋白分子核移位过程的检测
- Author:
Kai YANG
- Publication Type:Journal Article
- Keywords:
Semiconductor quantum dots;
Nanotechnology;
Dental papillae cells;
Smad4
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare semiconductor quantum dots (QDs)-Smad4 monoclonal antibody Fluorescent probes ,and to detect nucleation shifting process of Smad4 signal protein in Rat dental papillae cells(RDPCs) stimulated by TGF-?1 with the Fluorescent probes. Methods:①QDs were chemically modified with Smad4 proteins to prepare water soluble QDs-Smad4 monoclonal antibody Fluorescent probes which were purified after the preparation. ②The properties of these probes were studied through absorption band analyses,fluorescence spectra,fluorescence spectrum analyses and confocal laser scanning fluorescence microscopy. ③The location of Smad4 proteins in RDPCs was studied with SP anti-Smad4 immunocytochemical method,QDs direct mark method,Smad4 monoclonal antibody direct mark method,and direct immunofluorescence imaging.And the properties of Quantum Dots-Smad4 monoclonal antibody Fluorescent probes in RDPCs were detected. ④After RDPCs ware cultivated by TGF-?1 for 0 hours,12 hours,and 14 hours,the dynamic nucleation shifting process of Smad4 signal protein in RDPCs was separately detected with SP anti-Smad4 immunocytochemical method and direct immunofluorescence imaging.Results:QDs and monoclonal antibody ware covalently bonded to form the fluorescent probes which could specifically and effectively recognize Smad4 proteins in RDPCs .It is to demonstrate the dynamic nucleation shifting process of Smad4 signal protein in RDPCs. These Fluorescent probes still had good properties,including broad excite spectra,narrow emission spectra,high fluorescence intensity,and photostability. Conclusion:QDs and monoclonal antibody could be covalently bonded to form the fluorescent probes with distinct optics character,special immunity recognition capability and the ability of imaging mark the protein in cells for long time,which provides the scientific evidence that QDs can visually research the movement and interaction of protein in living cell .
