Expression of E-selectin and ICAM-1 in the healing process of incised wounds of rats skin and its significance in forensic medicine
- VernacularTitle:大鼠皮肤切创愈合过程中E-选择素及ICAM-1表达及法医学意义
- Author:
Ping WANG
- Publication Type:Journal Article
- Keywords:
E-selectin;
ICAM-1;
Skin incised injury;
Timing of wound age;
Immunohistochemistry
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression regulation of E-selectin and intercellular adhesion molecule-1(ICAM-1) in the heading process of incised wounds of rat skin and its significance in forensic medicine. Methods: After establ ishment of model,a study on the expression of E-selectin and ICAM-1 in vital rats skin incised wounds at different times were performed by immunohistochemistry technique,and evaluated with image analysis technique and statistic system. Results:There was no expre -ssion of E-selectin in the skin of normal control group. Positive expression of E-selectin could be observed on epidermis cells surface around injury 1h after injury.The expression level of E-selectin reached its peak on day 1 and then started to decline,lasted till day 7.The ratios of E-selectin positive vascular endothelial cells changed regularly according to different time after wound.In the vital skin incisions,while positive stain of ICAM-1 stain was observed on vascular endothelium as early as 1h after incision and lasted till 3d.In addition,the expression of ICAM-1 was also found in the inflammatory cells and fibroblasts and on vascular endothelium surface around injury,the lasting period varied from group to group There were no positive expression of E-selectin and ICAM-1 in the postmortem incision group.Conclusion:E-selectin and ICAM-1 are considered potentially useful markers for timing of wound age determination in forensic practice.
