Effect of curcumin on the activity of hepatic nuclear factor-kappa B and expression of peroxisome proliferator-activated receptor-? in rat with liver fibrosis induced by carbon tetrachoride
- VernacularTitle:姜黄素对四氯化碳诱导肝纤维化大鼠肝组织核因子-?B和过氧化物酶体增殖物激活受体?表达的影响
- Author:
Hang HE
;
Haiying HUA
;
Shiwen GE
- Publication Type:Journal Article
- Keywords:
curcumin;
liver fibrosis;
nuclear factor-kappa B;
peroxisome proliferato-?-activated receptor gamma
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of curcumin on the binding activity of hepatic nuclear factor-kappa B(NF-?B) and the expression of peroxisome proliferator-activated receptor-?(PPAR-?) in rats with liver fibrosis induced by carbon tetrachoride. METHODS: A total of 60 clean male rats were randomly and averagely divided into group A,B,C,D,E and F.The rats in group A served as normal controls,while those in other five groups were injected subcutaneously 40% CCI_4 for seven weeks to induce the model of liver fibrosis.After seven weeks,the rats in group C,D,E,F were intragastrically administered with 50 mg/kg silibinin,100 mg/kg cur,200 mg/kg cur,400 mg/kg cur once per day for six weeks,respectively.HE staining was used to observe the pathological changes of liver tissues under light microscope,and immunohistochemistry and reverse transcriptase polymerase chain reaction(RT-PCR) were performed to detect the activity of NF-?B,PPAR-? and mRNA expression of PPAR-?.RESULTS: The inflammatory and fibrotic degrees were obviously alleviated in group C,D,and E compared with group B.The expression of NF-?B p65 was significantly decreased in liver tissue in group C,D and E,compared with model control group B(P
